Taxonomic Contributions on Macrogynoplax Enderlein and Enderleina Jewett (Plecoptera: Perlidae) from Brazil, Including a New Species Supported by Molecular and Morphological Data.

Stoneflies (Plecoptera) are aquatic insects widely used in ecological and monitoring studies as indicators of environmental quality due to their high sensitivity to pollution. Among Brazilian Plecoptera, Perlidae is the most diverse family, with four genera (Anacroneuria Klapálek 1909, Enderleina Jewett 1960, Kempnyia Klapálek 1914, and Macrogynoplax Enderlein 1909) and about 150 species. Herein, based on data from the barcode region of the mitochondrial cytochrome c oxidase subunit I (COI) gene and morphological characters, we studied specimens of Enderleina and Macrogynoplax, mainly from the Brazilian Savanna. Our alignment with a total length of 608 base pairs included 23 COI sequences, of which 10 are new and 13 were obtained from GenBank. The sequences and congruence of the obtained species clusters were analyzed using neighbor joining. As a result, we describe a new species of Enderleina, the first of this genus in the Brazilian Savanna, and update the geographic distribution of some Macrogynoplax species. A new record was included for Macrogynoplax veneranda Froehlich 1984 in São Paulo state and the first record of M. yupanqui Stark 1996 in Brazil was also included. First records were made for Macrogynoplax matogrossensis Bispo and Neves 2005 and a Macrogynoplax specimen in Goiás and Bahia states, respectively. Consequently, we expanded the distribution of the genus in the Brazilian Savanna and Atlantic Forest. In addition, we provide observations on the morphological differences found among these species, discuss about the distribution of the genera, present molecular data discussing their contributions to the knowledge of the genus, and comment on the recommendations for future research considering a scenario of integrative approaches. Lastly, we present a key to identify the adult males of Enderleina.

Anacroneuria (Plecoptera: Perlidae) from Central Brazil: new species, new geographic records, and taxonomic notes.

In the present study, adult specimens of Anacroneuria collected in streams were studied in order to understand the diversity of the genus in Central Brazil. Herein, a new species (A. froehlichi n. sp.) is described, new geographic occurrences are recorded (A. amargosa Righi-Cavallaro Froehlich, 2013; A. mineira Novaes Bispo, 2014; A. minuta Klaplek, 1922; A. ofaye Froehlich, 2007; A. payagua Froehlich, 2007; A. singela Duarte Lecci, 2016; and A. singularis Righi-Cavallaro Lecci, 2010), and taxonomic comments are made. Additionally, photographs of the paratypes of A. dourada Jewett, 1960 and A. galba Jewett, 1960 are presented. A total of 16 species of Anacroneuria are now recorded in Central Brazil.

Perlidae (Plecoptera) from Bahia State, Brazil: description of the nymphs of Anacroneuria bahiensis Righi-Cavallaro amp; Lecci, notes on Anacroneuria debilis (Pictet), and updated list of the species.

In the present study, specimens of Anacroneuria (Plecoptera: Perlidae) from the Mucug Municipal Park, Bahia State, Brazil were studied. The association between Anacroneuria bahiensis nymph and adult was possible because a specimen, as well as the exuviae, was collected during its emergence. Herein, we present the description of the A. bahiensis nymph, taxonomic notes on A. debilis, and an updated list of Anacroneuria species from Bahia State.

The genus Anacroneuria Klapálek, 1909 (Plecoptera: Perlidae) from Maranhão State, northeastern Brazil.

Based on collections made from streams of the Itapecuru, Parnaíba and Tocantins River basins, three species of Anacroneuria Klapálek are recorded for the first time from Maranhão State, northeastern Brazil: A. atrifrons Klapálek, A. minuta Klapálek and A. singularis Righi-Cavallaro Lecci. Additionally, A. leccii sp. nov. is described from this material and remarks concerning A. marlieri are also presented.

Anacroneuria iporanga (Plecoptera: Perlidae): description of the nymph and biological notes.

The Neotropical stonefly genus Anacroneuria (Perlidae) is represented by approximately 80 species in Brazil, but the nymphs of only 11 of these species have been associated and described. In this paper, we describe the previously unknown nymph of A. iporanga from Intervales State Park, São Paulo State, Brazil and present biological observations for this species.